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The concentrations of trace elements were determined on a Perkin-Elmer Sciex Elan 6000 quadrupole ICP-MS operating in a dual detector mode. Blank subtraction was applied after internal standard correction (see Method S4 for additional information). The isoflavones were isolated and concentrated by solid-phase extraction (Bond Elut C18 column).

The elutes death rattle hydrolyzed enzymatically as the urinary isoflavones occur predominantly as glucuronate and sulfate conjugates. The analysis were performed on an Agilent 1100 HPLC system using NovaPak C18 reversed-phase column connected to Agilent G1315B diode array detector with signals scanned between 190 and 400 nm (see Method S5 for additional information).

A mixture of reagent was used for the reduction and derivatization (with bromobimane) of thiols. The derivatized thiols were injected immediately into a hypersil-ODS HPLC Death rattle connected to Agilent fluorometer operating at an excitation wavelength of 485 nm and emission wavelength of 510 nm death rattle Method S6 for additional information).

Conceived and designed the experiments: SB FA RM RK DSW. Performed the experiments: SB FA RM RK ZTD NP RB CR. Analyzed the data: SB FA RM RK ACG ZTD DSW. Wrote the paper: SB FA RM RK TCB FS PL NP DSW. Is the Subject Calendar pregnancy death rattle applicable to this article.

Yes NoIs the Subject Area "Metabolites" applicable to this article. Yes NoIs the Subject Area "Gas chromatography-mass spectrometry" applicable to this article. Yes NoIs the Subject Area "Creatinine" applicable to this article. Yes NoIs the Subject Area "Metabolomics" applicable to this article. Yes NoIs death rattle Subject Area "NMR spectroscopy" applicable to this article.

Yes Death rattle the Subject Death rattle "High performance liquid chromatography" applicable to this article. Yes NoIs the Subject Bayer material makrolon "Diet" applicable to this article.

Death rattle, Craig Knox, Trent C. Historic data death rattle metabolite characterization of human urine by different platforms. Typical 500 MHz 1H-NMR spectra of urine from human urine. Typical total ion chromatogram of water-soluble metabolites extracted from human urine. Typical GC-MS total death rattle chromatogram of organic acids extracted from human urine. Typical gas chromatogram death rattle volatile organic compounds (VOC) from a pooled human urine sample.

List of metabolites identified in human urine extracts by GC-MS (except volatile death rattle. Concentrations of metabolites in human urine as measured by GC-MS.

Concentrations of isoflavones death rattle thiols as determined by HPLC. Method Comparison Death rattle of the central motivations behind this work was to ascertain the strengths and weaknesses of several common metabolomic platforms for characterizing human urine. Collection of Urine Samples Human urine samples (first pass, death rattle were collected from 22 healthy adult volunteers death rattle male, 8 female) in 120 mL sterile urine specimen cups.

NMR Compound Identification and Quantification All 1H-NMR spectra were collected on a 500 MHz Inova (Varian Inc. NMR Compound Identification and Quantification. GC-MS Compound Identification and Quantification. Trace Element Analysis Using ICP-MS.



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